You HAVE to get a custom tune for E85. Running E85 in a vehicle that cannot compensate for the MANDATORY decrease in air/fuel ratio will cause codes (P0171/P0174), lack of power (more than you'll lose just going to E85 with dedicated tuning), and driveability concerns.
E85 has a stoichiometric ratio of around 9.6:1. Gasoline's stoich ratio is 14.7:1. The engine control module is only able to compensate so much for a perceived lean condition.
It takes A LOT more E85 per unit of air to maintain happy operation than with gasoline parameters.
Is there any particular reason that you are wanting to run E85?
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